We narrowed to 14,099 results for: cas9

1. pAT-9208

   Plasmid

   #124221

   Purpose

   Cloning plasmid for a self-targeting gRNA library

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. gh124

   Plasmid

   #106794

   Purpose

   expression of gRNA targeting ST8SIA4

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ST8SIA4 (ST8SIA4 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

   Plasmid

   #85749

   Purpose

   Expresses anti-CRISPR protein from Type I-E and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type I-E anti-CRISPR
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_NANOG

   Plasmid

   #64153

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh87

   Plasmid

   #106758

   Purpose

   expression of gRNA targeting FUT3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   FUT3 (FUT3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEasyG3_zeo

    Plasmid

    #184917

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD1CB0202

    Plasmid

    #185626

    Purpose

    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid

    #140204

    Purpose

    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Step 1 of cloning c…

    Promoter

    Aspergillus fumigatus U3 (RNAPIII).

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. gh172

    Plasmid

    #106833

    Purpose

    expression of gRNA targeting UGGT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT1 (UGGT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. gh121

    Plasmid

    #106780

    Purpose

    expression of gRNA targeting ST8SIA1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST8SIA1 (ST8SIA1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh49

    Plasmid

    #106722

    Purpose

    expression of gRNA targeting ST3GAL1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST3GAL1 (ST3GAL1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh42

    Plasmid

    #106716

    Purpose

    expression of gRNA targeting ST6GALNAC1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST6GALNAC1 (ST6GALNAC1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. gh45

    Plasmid

    #106708

    Purpose

    expression of gRNA targeting ST6GALNAC4

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST6GALNAC4 (ST6GALNAC4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124545

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIK137

    Plasmid

    #65632

    Purpose

    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    

    Expression

    Worm

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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