We narrowed to 7,494 results for: tet on

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pCI-neo-His-MS2BP-G3BP1-WT

Plasmid

#136008

Purpose

WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

MS2BP

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBW313lux-hrpR

Plasmid

#61436

Purpose

pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

F2620-rbs33-hrpR-ter

Expression

Bacterial

Promoter

plux

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mTq2

Plasmid

#67031

Purpose

Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT5

Plasmid

#53560

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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IBMc071

Plasmid

#161943

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTL-Bag3-gRNA3

Plasmid

#196254

Purpose

Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2-gRNA2

Plasmid

#196252

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag1-gRNA1

Plasmid

#196251

Purpose

Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag4-gRNA4

Plasmid

#196255

Purpose

Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-Bag2T-gRNA2

Plasmid

#196253

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFAB822

Plasmid

#47856

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO11

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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p5B3-T

Plasmid

#92021

Purpose

Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lysA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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