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YIplac211-VPS8-iGFPx6

Plasmid

#105271

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Trpc2-probe2

Plasmid

#105474

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Cell Rep. 2014 Jul 24;8(2):583-95. doi:

Insert

Trpc2 (Trpc2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Construct 7 - CMVp-Cas9-3xNLS-HSVpA

Plasmid

#81247

Purpose

Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Expression

Mammalian

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP-VPS21

Plasmid

#105265

Purpose

Plasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps21 (VPS21 Budding Yeast)

Tags

msGFP

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SNX4-iGFPx3

Plasmid

#105269

Purpose

Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snx4 portion (SNX4 Budding Yeast)

Tags

iGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP2

Plasmid

#51885

Purpose

expression of Xl-VSP2 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-VP64-SeqFosA

Plasmid

#51430

Purpose

Artificial transcription factor with VP64 targeting c-Fos (TALE A)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosA

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn3

Plasmid

#61875

Purpose

bacterial expression of a GST-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP_mNTAQ1

Plasmid

#65486

Purpose

Mouse Ntaq1-EGFP reporter

Depositor

Alexander Varshavsky

Article

Wang et al Mol Cell. 2009 Jun 26;34(6):686-95. doi:

Insert

Ntaq1 (Wdyhv1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pUC57-FASTKD4

Plasmid

#73620

Purpose

Cloning vector containing FASTKD4

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

FASTKD4 has been codon optimized for expression i…

Promoter

lac

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
YIplac204-T|C-iGFP-RCY1

Plasmid

#105281

Purpose

Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Rcy1 (RCY1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Promoter

TPI1

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEG607

Plasmid

#40193

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

Expression

Worm

Promoter

4.4 kb mex-5 promoter fragment

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Spindly

Plasmid

#24282

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Spindly (Spindly Fly)

Tags

GFP

Expression

Insect

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pAF1-VP16-SeqFosB

Plasmid

#51431

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE B)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosB

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pQuantFLAG-bsr-kana

Plasmid

#45593

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-FLAG tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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