We narrowed to 9,277 results for: Pol;

1. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

   Plasmid

   #111389

   Purpose

   AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   ChETA-mRuby2
   W3SL
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   W3SL is built from a shortened WPRE, an upstream …

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2273

   Plasmid

   #111899

   Purpose

   ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   AAV

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1α-BHLHE40-IRES-ZsGreen1

   Plasmid

   #153061

   Purpose

   Lentiviral vector expressing human BHLHE40

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Duet-1-NONO(53-312)

   Plasmid

   #135442

   Purpose

   Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   Non-POU domain-containing octamer-binding protein (NONO Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+/-FLI1-C‐(K)‐DYK

   Plasmid

   #129497

   Purpose

   Mammalian expression of human FLI1

   Depositor

   Marjorie Brand

   Article

   Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

   Insert

   FLI1 (FLI1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPRv2_sgCtrl#1

   Plasmid

   #174148

   Purpose

   Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. DENV4 Rluc Reporter GND mutant Replicon

   Plasmid

   #118586

   Purpose

   For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

   Depositor

   Radhakrishnan Padmanabhan

   Article

   Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

   Insert

   DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
   

   Use

   Yeast-e. col shuttle vector prs424

   Mutation

   The conserved GDD in the NS5 polymerase gene muta…

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PH-Btk-mRuby2

   Plasmid

   #124657

   Purpose

   PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   BTK (BTK Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   contains only the PH domain of Bruton tyrosine ki…

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Trim46

   Plasmid

   #176402

   Purpose

   TRIM46 tagged with GFP

   Depositor

   Casper Hoogenraad, Thomas Schwarz

   Article

   van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

   Insert

   trim46 (Trim46 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1deltaART

    Plasmid

    #174804

    Purpose

    Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1deltaART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 785-1014

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2280

    Plasmid

    #111901

    Purpose

    ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple345 (NEFL Human)
    

    Use

    AAV

    Promoter

    Ple345 (NEFL MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1delZn2delBRCT

    Plasmid

    #173939

    Purpose

    Bacterial expression of PARP1 lacking Zn2 and BRCT domains

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2delBRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 and 374-520; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Trim46

    Plasmid

    #176401

    Purpose

    TRIM46 tagged with mCherry

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACTB-(A)4x

    Plasmid

    #112058

    Purpose

    ACTB mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKHH030_sgCtrl#1

    Plasmid

    #174142

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-EGFR*-CUbo

    Plasmid

    #212821

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Tags

    CUbo

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mouse av full length

    Plasmid

    #217809

    Purpose

    Full length mouse integrin av expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGAV (Itgav Mouse)
    

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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