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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,991 results for: LEA

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Showing: 6561 - 6580 of 14991 results

  2. UAS:bloSwitch

    Plasmid
    #108355
    Purpose
    UAS driven green to red switch reporter for B3 recombinase expression
    Depositor
    Harold Burgess
    Publication
    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.
    Insert
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    Use
    Zebrafish expression
    Mutation
    Zebrafish codon optimized
    Promoter
    lac
    Available since
    Sept. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    108355-plasmid-map-sequence-id-208272

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  3. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  4. TBXTA-c028

    Plasmid
    #139765
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, WT (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139765-plasmid-map-sequence-id-296580

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  5. pGBW-m4046230

    Plasmid
    #145454
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145454-plasmid-map-sequence-id-300252

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  13. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pEMS1506

    Plasmid
    #29251
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple35 (CLDN5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29251-plasmid-map-sequence-id-13666

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  16. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. Dronpa-Nucleus-9

    Plasmid
    #57290
    Purpose
    Localization: Nucleus, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    Dronpa-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57290-plasmid-map-sequence-id-183119

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Showing: 6561 - 6580 of 14991 results