We narrowed to 24,157 results for: LEA

1. pLU-CMV-Flag-hATG5 E122D

   Plasmid

   #111804

   Purpose

   Mammalian Expression of hATG5

   Depositor

   Jun Hee Lee

   Article

   Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

   Insert

   hATG5 (ATG5 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E122D

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPc1 hp47phox (S303/304/328D)

   Plasmid

   #112245

   Purpose

   expression of mutant p47phox

   Depositor

   Simone Di Giovanni

   Article

   Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

   Insert

   p47phox (NCF1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S303/304/328D

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH1/M-FGFR3-Fv-Fvls-E

   Plasmid

   #15287

   Depositor

   David Spencer

   Article

   Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

   Insert

   FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
   

   Tags

   Myristoylation-targeting domain c-Src (14aa) and …

   Expression

   Mammalian

   Mutation

   FGFR3 cytoplasmic domain only FKBP12 F36V

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSEss-CHIg-mM-Asn209Asp_M18

   Plasmid

   #91781

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

   Insert

   immunoglobulin heavy constant mu (Ighm Mouse)
   

   Expression

   Mammalian

   Mutation

   Asn209 changed to Asp (EU numbering)

   Promoter

   hEF1-HTLV prom

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-hPC1_4106-eGFP-3HA

   Plasmid

   #108399

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-CD16.7-hPC1-eGFP-3HA

   Plasmid

   #108401

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-SM

   Plasmid

   #136578

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2-E2A-cMyc (Sox2, Myc Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ISG15 C-term. Domain intein/chitin binding domain (79-156)

   Plasmid

   #110760

   Purpose

   Bacterial expression of ISG15 C-term.

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Tags

   intein/chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

   Plasmid

   #99746

   Purpose

   Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Made six (6) silent mutations to confer resistanc…

   Promoter

   CMV/TO

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pelB-MBP-RevA-ss

    Plasmid

    #137064

    Purpose

    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    lacks the RevA signal peptide and 5 residues (CKA…

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pelB-MBP-DipA-ss

    Plasmid

    #137068

    Purpose

    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature DipA: lacks the DipA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Mutation

    H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid

    #91733

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-hAR-R617M,K618M,K632M,K633M

    Plasmid

    #115778

    Purpose

    Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    Androgen Receptor (AR Human)
    

    Expression

    Mammalian

    Mutation

    R617M, K618M ,K632M, K633M, insertion of Q81 and …

    Promoter

    CMV

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-Flag-SPRTN-Y117C

    Plasmid

    #110216

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y117C, P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid

    #112713

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    BirA* (BirA R118G), HIV Rev nuclear localization …

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

    Plasmid

    #110761

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    intein/chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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