We narrowed to 9,753 results for: try

1. pDONR P2R-P3-EYFP

   Plasmid

   #48351

   Purpose

   Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway entry vector

   Mutation

   Contains a stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. eDrIR1

   Plasmid

   #183998

   Purpose

   Optically controlled eDrIR1 expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrIR1 (INSR Human)
   

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR E2 HSPter-35Sp-mito

   Plasmid

   #158727

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   A terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
   

   Use

   Other

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-H2B-mCardinal (JDW 1418)

   Plasmid

   #242553

   Purpose

   Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCardinal
   

   Use

   Gateway subcloning

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-4xTR-TUBE

   Plasmid

   #110312

   Purpose

   E. coli expression and purification of 4xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   4xTR-TUBE (UBQLN1 Human)
   

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1777 (pEffector)

   Plasmid

   #160731

   Purpose

   Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins and crRNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p221a-GUS

   Plasmid

   #71263

   Purpose

   Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ Grx1-roCherry

    Plasmid

    #141068

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threon…

    Promoter

    CMV, SP6

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1 Grx1-roCherry-mito

    Plasmid

    #141069

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Tags

    dMTS

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threoni…

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4-NHK-MYC-His6

    Plasmid

    #185350

    Purpose

    Assessing of ubiquitination in cells

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    a1-antitrypsin Hong Kong Null mutant
    

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    NHK mutant

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1524

    Plasmid

    #111431

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8138-E02

    Plasmid

    #225659

    Purpose

    Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    SORE6
    

    Use

    Gateway entry clone

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. amilCP_Pink chromoprotein

    Plasmid

    #117851

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Pink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    64A,65C, BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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