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We narrowed to 2,823 results for: cdna expression plasmid

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Showing: 1601 - 1650 of 2823 results

  1. ACE2-Mutant-C4

    Plasmid
    #240608
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240608-plasmid-map-sequence-id-483854

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  2. ACE2-Mutant-C(3+4)

    Plasmid
    #240611
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240611-plasmid-map-sequence-id-482833

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  3. ACE2-Mutant-C(0+4)

    Plasmid
    #240610
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240610-plasmid-map-sequence-id-482820

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  6. pAc5.1B-EGFP-DmPABPC1-V5His6_G

    Plasmid
    #146352
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Expression
    Insect
    Mutation
    one non silent mutation G26S, and seven silent mu…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146352-plasmid-map-sequence-id-288797

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  18. LentiV_Neo_LKB1_CR

    Plasmid
    #108112
    Purpose
    Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    LKB1 (STK11 Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    change cytosine 333 to alanine (silent mutation)
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108112-plasmid-map-sequence-id-207845

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  19. pAc5.1B-EGFP-DmFMR1_B

    Plasmid
    #145920
    Purpose
    Insect Expression of DmFMR1
    Depositor
    Elisa Izaurralde
    Insert
    DmFMR1 (Fmr1 Fly)
    Expression
    Insect
    Mutation
    Two amino acid mutations (T214I and N501S) and th…
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145920-plasmid-map-sequence-id-368862

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  20. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid
    #145940
    Purpose
    Insect Expression of DmFMR1
    Depositor
    Elisa Izaurralde
    Insert
    DmFMR1 (Fmr1 Fly)
    Expression
    Insect
    Mutation
    Two amino acid mutations (N45D and G558R) and thr…
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145940-plasmid-map-sequence-id-368870

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  23. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  24. STING-V2

    Plasmid
    #124264
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124264-plasmid-map-sequence-id-241461

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  25. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

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  26. V2-STING

    Plasmid
    #124265
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus2
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124265-plasmid-map-sequence-id-241431

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  28. AT1R-HA

    Plasmid
    #240614
    Purpose
    Encodes AT1R, a key receptor on RAAS that regulated blood pressure
    Depositor
    Zaid Abassi
    Insert
    Angiotensin II Type 1 Receptor (AGTR1 Human)
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    240614-plasmid-map-sequence-id-483856

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  30. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  31. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  32. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114193-plasmid-map-sequence-id-224265

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  33. Epac-S-H127

    Plasmid
    #170343
    Purpose
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170343-plasmid-map-sequence-id-344642

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  40. HA-hDAGLb-V5

    Plasmid
    #87675
    Purpose
    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Insert
    Human Diacylglycerol Lipase Beta (DAGLB Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87675-plasmid-map-sequence-id-173605

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  43. LentiV_Neo_SIK3_CR_K95M

    Plasmid
    #108106
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108106-plasmid-map-sequence-id-207840

    Enlarge

  44. LentiV_Neo_SIK3_CR_T221E

    Plasmid
    #108108
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108108-plasmid-map-sequence-id-207841

    Enlarge

  47. LentiV_Neo_SIK3_CR_T221A

    Plasmid
    #108107
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108107-plasmid-map-sequence-id-210058

    Enlarge

  49. LentiV_Neo_SIK3_CR_T142Q

    Plasmid
    #108109
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108109-plasmid-map-sequence-id-207842

    Enlarge

Showing: 1601 - 1650 of 2823 results