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pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162386

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162388

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBX_RPS2_smGFP_V5

Plasmid

#236088

Purpose

Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS2-smGFP-V5 (RPS2 Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBX_Sec61b_smGFP_Myc

Plasmid

#236092

Purpose

Constitutively encodes Sec61b-smGFP-Myc (sfGFP-based spaghetti monster fluorescent protein embedding Myc epitope tags), for targeting the endoplasmic reticulum.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Sec61b-smGFP-Myc (SEC61B Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162384

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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CALCRL-Tango

Plasmid

#66234

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CALCRL (CALCRL Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162383

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2

Plasmid

#86237

Purpose

Lentivirus for expression of human Plexin-B2

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

full length PLXNB2, CRISPR resistant

Promoter

human PGK promoter

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-GST-Tev-Af1521-myc

Plasmid

#196239

Purpose

N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

N-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag

Tags

GST tag and myc tag

Expression

Bacterial

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C3AR1-Tango

Plasmid

#66231

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

C3AR1 (C3AR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-TOMM20-N-10

Plasmid

#56239

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_014765.2 (TOMM20 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA4.TO-ORF73-2xCSTREP

Plasmid

#136232

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF73

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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BDKBR2-Tango

Plasmid

#66230

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

BDKBR2 (BDKRB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1859A

Plasmid

#46239

Purpose

FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1859 changed to Alanine (S1859A)

Promoter

cmv

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
C5A-Tango

Plasmid

#66232

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

C5A (C5AR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pNOC_hfnCas12a-Nlux

Plasmid

#176239

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CCR10-Tango

Plasmid

#66238

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCR10 (CCR10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
CALCRb-Tango

Plasmid

#66233

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CALCRb (CALCRL Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
CCKBR-Tango

Plasmid

#66237

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCKBR (CCKBR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only