-
Plasmid#202205PurposeExpresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cellsDepositorInsert[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GalT-moxDendra2
Plasmid#89789Purposemammalian expression of GC localized moxDendra2DepositorInsertGalT signal anchor transmembrane domain (B4GALT1 Human)
UseTagsmoxDendra2ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-MTS-FLAG-LOV*
Plasmid#202208PurposePlasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cellsDepositorInsertCytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
UseLentiviralTagsFLAG and LOV*ExpressionMutationPromoterEf1aAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZE46 DeltaPGB Dvl2 pCS2P+
Plasmid#17087DepositorInsertDvl-2 (dvl2 Zebrafish)
UseZebrafish expressionTagsExpressionMutationDeltaPGB (deletes amino acids 231-252)PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2
Plasmid#48146PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1
Plasmid#48145PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
IKK-2 K44M
Plasmid#11104DepositorInsertIKK-2 (IKBKB Human)
UseTagsFLAGExpressionMammalianMutationK44M, kinase inactivePromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
ECFP-IKB
Plasmid#111193Purposefluorescent fusion proteinDepositorInsertIKB-alpha
UseTagsECFPExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-NFKB1
Plasmid#139822Purposedoxycycline-inducible expression of Human NFKB1 in mammalian cellsDepositorInsertNFKB1 (NFKB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVTagsExpressionMammalianMutationPromoterCAG, H1, and U6Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
TS-PE2-N
Plasmid#176649PurposeExpresses N-terminus of PE2 in mammalian cellsDepositorInsertsN-terminus of PE2
Artificial SD
UseAAVTagsSV40 NLSExpressionMammalianMutationPromoterCMV and NoneAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EYFP-IKB
Plasmid#111194Purposefluorescent fusion proteinDepositorInsertIKB-alpha
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC-NF8-d1-eGFP
Plasmid#122036PurposeAAV vector, NFkappaB-dependent eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only