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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,138 results for: Tef

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Showing: 641 - 660 of 1138 results

  1. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Expression
    Yeast
    Mutation
    DHFR P66L
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  2. pIRGAL43C

    Plasmid
    #218278
    Purpose
    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218278-plasmid-map-sequence-id-433680

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  4. pIRHDGAL3C

    Plasmid
    #218276
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218276-plasmid-map-sequence-id-433677

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  5. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Claudia Vickers
    Article
    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185869-plasmid-map-sequence-id-370814

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  7. pJF16

    Plasmid
    #253747
    Purpose
    Encodes GFP_AID* and pALD6-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    ALD6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253747-plasmid-map-sequence-id-507847

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  8. pJF15

    Plasmid
    #253746
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RPL18B and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253746-plasmid-map-sequence-id-507840

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  9. pJF17

    Plasmid
    #253748
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    RNR2 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253748-plasmid-map-sequence-id-507839

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  10. pJF18

    Plasmid
    #253749
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    REV1 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253749-plasmid-map-sequence-id-507834

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  11. pJF19

    Plasmid
    #253750
    Purpose
    Encodes GFP_AID* and pSAC6-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    SAC6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253750-plasmid-map-sequence-id-507833

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  12. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  13. pIRHDGAL43C

    Plasmid
    #218279
    Purpose
    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction
    Depositor
    Claudia Vickers
    Article
    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.
    Insert
    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    Expression
    Yeast
    Mutation
    GAL3 (S509P), DHFR (P66L)
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218279-plasmid-map-sequence-id-433681

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  15. pJF89

    Plasmid
    #253756
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RNR2 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253756-plasmid-map-sequence-id-509520

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  16. pJF90

    Plasmid
    #253757
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    REV1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253757-plasmid-map-sequence-id-509519

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  17. pJF91

    Plasmid
    #253758
    Purpose
    Encodes GFP_AID* and pADH1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    ADH1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253758-plasmid-map-sequence-id-509518

    Enlarge

  18. pJF86

    Plasmid
    #253753
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RPL18B and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253753-plasmid-map-sequence-id-509401

    Enlarge

  19. pJF88

    Plasmid
    #253755
    Purpose
    Encodes GFP_AID* and pSAC6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    SAC6 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253755-plasmid-map-sequence-id-509369

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  20. pJF34

    Plasmid
    #253752
    Purpose
    Encodes GFP_AID* and pADH1-OsTIR1(F74G) expression cassettes. The OsTIR1(F74G) coding sequence is not codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1 (non codon optimized)
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G
    Promoter
    ADH1 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253752-plasmid-map-sequence-id-507841

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Showing: 641 - 660 of 1138 results