We narrowed to 1,247 results for: ncl
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Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneExpressionBacterialPromotergapDH promoter from Eggerthella lentaAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only
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pLBH546_Tet-Cas14b1Locus
Plasmid#112503PurposeExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b1
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH547_Tet-Cas14b2Locus
Plasmid#112504PurposeExpresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b2
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
Tagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialPromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2AGAL4
Plasmid#125211PurposeArtificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2AGAL4
Plasmid#125212PurposeArtificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL4
Plasmid#125213PurposeArtificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Halo-Erk2 donor
Plasmid#113104PurposeDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequenceDepositorInsertN terminal Erk2 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
KG#45
Plasmid#110926PurposeVector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegansDepositorInsertshsp-16-2 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
TagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Id2-Halo donor
Plasmid#113116PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequenceDepositorInsertId2 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2AGeneSwitch
Plasmid#125214PurposeArtificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSETT4tip
Plasmid#153414PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneExpressionBacterialPromotertipA promoter from Streptomyces lividansAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLY108
Plasmid#130938PurposeEngineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB1
UseSynthetic BiologyExpressionBacterialPromoterPlux2Available SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSA-38
Plasmid#17328DepositorInsertstreptavidin
ExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…Available SinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDH5
Plasmid#83775PurposeUseful for tagging yeast proteins with C-terminal YFP tag; his selectionDepositorInsertYFP
TagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only