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pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

Plasmid

#227159

Purpose

For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-halo-his

Plasmid

#227161

Purpose

For T-REX experiments of CYP2A6 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV pSYN eGFP T2A SP HALO HAPLN1

Plasmid

#228199

Purpose

AAV vector expressing GFP and HaloTag-HAPLN1 under synaptophysin promoter

Depositor

Sungjin Park

Article

Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

Insert

HAPLN1 (Hapln1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human synaptophysin promoter

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHThMeCP2 (pc3972)

Plasmid

#227639

Purpose

Mammalian expression vector for human MeCP2 C-terminally tagged to HaloTag.

Depositor

Cristina Cardoso

Article

Schmidt et al Cells. 2024 Jan 11;13(2):139. doi: 10.33

Insert

MeCP2 (MECP2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-Sec61b

Plasmid

#210290

Purpose

nucleus OM marker and "anchor" for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-Sec61b

Tags

ECFP and FRB

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Tom20-EYFP-miLID

Plasmid

#210291

Purpose

mitochondria marker and "anchor" for optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

Tom20-EYFP-miLID

Tags

EYFP and iLID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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mActA-mSspB-mCherry

Plasmid

#210294

Purpose

ActuAtor for iLID optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-,SspB-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-GiantinS

Plasmid

#210296

Purpose

Golgi marker and anchor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-GiantinS

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-DN-N-WASP

Plasmid

#210301

Purpose

control for targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-DN-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Article

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTij_sg_mmu_Med1_N_terminal

Plasmid

#197870

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pST_AfLEA1.1 (pBS0741)

Plasmid

#185233

Purpose

For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfLEA1.1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_HYPDU (pBS0650)

Plasmid

#185194

Purpose

For the mammalian expression of the tardigrade protein CAHS1_HYPDU. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_HYPDU

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_HUMAN (pBS0645)

Plasmid

#185190

Purpose

For the mammalian expression of the human protein APOA4_HUMAN. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_HUMAN

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A0A1D1UKG0_RAMVA (pBS0352)

Plasmid

#185188

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UKG0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UKG0_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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