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non-targeting control gRNA (BRDN0001148372)

Plasmid

#80258

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK4 gRNA (BRDN0001145019)

Plasmid

#76560

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ABE8e(TadA-8e K20A R21A)

Plasmid

#138496

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e K20A R21A)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e K20A R21A

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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RPS6KA1 gRNA (BRDN0001148481)

Plasmid

#75499

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIK2 gRNA (BRDN0001147148)

Plasmid

#77140

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK2 (SIK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTBK2 gRNA (BRDN0001147885)

Plasmid

#77968

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTBK2 (TTBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(m40)-luc

Plasmid

#12425

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

PU.1 binding site mutation (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
TOB2

Plasmid

#156090

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TOB2 (TOB2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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TOB1

Plasmid

#156089

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TOB1 (TOB1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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THOC6

Plasmid

#156085

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC6 (THOC6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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NAP1L3

Plasmid

#155763

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NAP1L3 (NAP1L3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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RY1

Plasmid

#155986

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RY1 (SNRNP27 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PNRC2

Plasmid

#155830

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PNRC2 (PNRC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PLRG1

Plasmid

#155827

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PLRG1 (PLRG1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147768)

Plasmid

#76194

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo-(HA)3-ε

Plasmid

#198531

Purpose

Expression of HA-tagged AP-4 ε in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

AP-4 ε (AP4E1 Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitutions in codons 235, 262, 905 and …

Promoter

CMV

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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RFK gRNA (BRDN0001146181)

Plasmid

#76847

Purpose

3rd generation lentiviral gRNA plasmid targeting human RFK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RFK (RFK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RFK gRNA (BRDN0001162491)

Plasmid

#76850

Purpose

3rd generation lentiviral gRNA plasmid targeting human RFK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RFK (RFK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ROCK2 gRNA (BRDN0001146481)

Plasmid

#76350

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK2 (ROCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001145885)

Plasmid

#80196

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge