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INCTbiosyn-pAct-AtINT9

Plasmid

#127533

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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PAK2 gRNA (BRDN0001147045)

Plasmid

#77567

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK2 (PAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK2 gRNA (BRDN0001148074)

Plasmid

#77568

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK2 (PAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK2 gRNA (BRDN0001146615)

Plasmid

#77569

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK2 (PAK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TP53RK gRNA (BRDN0001145338)

Plasmid

#77699

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TP53RK gRNA (BRDN0001146963)

Plasmid

#77700

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIM27 gRNA (BRDN0001148850)

Plasmid

#77517

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM27

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM27 (TRIM27 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001162227)

Plasmid

#76276

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001147750)

Plasmid

#77926

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

WT HEC1 sequence

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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cmv hTid Long H121Q

Plasmid

#13708

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long H121Q (DNAJA3 Human)

Expression

Mammalian

Mutation

H121Q

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
TVBB C-term-Dendra2

Plasmid

#169216

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148852)

Plasmid

#80175

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147812)

Plasmid

#80176

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAF161

Plasmid

#135926

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

TNFRSF9 fused to mCerulean3

Use

Transposon

Tags

mCerulean3

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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SRPK1 gRNA (BRDN0001147971)

Plasmid

#77492

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK1 (SRPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K2 gRNA (BRDN0001148469)

Plasmid

#76781

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K2 (MAP4K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ULK2 gRNA (BRDN0001145207)

Plasmid

#77260

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK2 (ULK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ULK2 gRNA (BRDN0001145025)

Plasmid

#77261

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK2 (ULK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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