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pBlastR-5xUAS-mCherry-MiniWhite

Plasmid

#165911

Purpose

Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-GAL4-MiniWhite

Plasmid

#165890

Purpose

Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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DT050A

Plasmid

#159411

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-NEK9W967A

Plasmid

#168271

Purpose

Expresses LIR (LC3-interacting region) mutant NEK9

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 979 to alanine

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

Plasmid

#114371

Purpose

expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-MBD (NEWENTRY )

Use

AAV

Tags

EYFP and MBD

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPK-351

Plasmid

#157921

Purpose

pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

Depositor

Phillip Kyriakakis

Article

Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

Inserts

PIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD

Use

Synthetic Biology; Optogenetics

Tags

HA tag and SV40NLS

Expression

Mammalian

Mutation

pif3 1-523

Promoter

CMV

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway entry vector

Promoter

H1, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-EBFP2-MiniWhite

Plasmid

#165918

Purpose

Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)

Use

Entr clone

Promoter

H1/TO

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pENTR4_FUS_C2G_R521G

Plasmid

#26369

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Mutation

R521G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pVL90 - VND7

Plasmid

#116035

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

VND7 (VND7 Mustard Weed)

Use

Unspecified

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUT1109-HQ858666

Plasmid

#27719

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

C2C2-CO-like transcription factor from DT643534

Use

Entry vector

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ255-x3.7

Plasmid

#124311

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A_x3.7-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ256-x3.7

Plasmid

#124313

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A_x3.7-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

Plasmid

#114374

Purpose

expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUT1141-HQ858685

Plasmid

#27731

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE179215

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1439-HQ858741

Plasmid

#27823

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DT947867

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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