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pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113342

Purpose

Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pNP555

Plasmid

#222722

Purpose

sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE3-4

Plasmid

#219946

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI826

Plasmid

#216634

Purpose

cpTurboID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_trigger

Plasmid

#132747

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_trigger

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX311 LacZ

Plasmid

#117731

Purpose

Open reading frame vector encoding LacZ

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

LacZ

Use

Lentiviral

Tags

2X V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-LbCas12a

Plasmid

#213743

Purpose

For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N19_switch

Plasmid

#132723

Purpose

T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAR3040-spitz

Plasmid

#38341

Depositor

Norbert Perrimon

Article

Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

Insert

spitz (spi Fly)

Expression

Bacterial

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_W267N

Plasmid

#214311

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

W267N

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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nGR2175-gs10-NLS-VPR

Plasmid

#188264

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nGR2175

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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