Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,126 results for: LEA

Showing: 6741 - 6760 of 12126 results

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-Cb5-pEGFP-C1

Plasmid

#166764

Purpose

To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

Depositor

David Andrews

Insert

Cb5 tail anchor (Cyb5a Rat)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc326

Plasmid

#161949

Purpose

Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Doc2alpha D291N mutant GFP

Plasmid

#128821

Purpose

To visualize Doc2alpha D291N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D291N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta D303N mutant GFP

Plasmid

#128822

Purpose

To visualize Doc2beta D303N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D303N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134411

Plasmid

#152311

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

Sept. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134524

Plasmid

#152298

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133500

Plasmid

#152972

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133669

Plasmid

#152930

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133409

Plasmid

#152854

Purpose

Mammalian expression plasmid for SARS-CoV-2 endoRNAse

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134439

Plasmid

#152833

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133585

Plasmid

#152820

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133540

Plasmid

#152813

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133535

Plasmid

#152801

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133405

Plasmid

#152802

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133966

Plasmid

#152719

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134448

Plasmid

#152714

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133861

Plasmid

#152698

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134517

Plasmid

#152659

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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