We narrowed to 7,272 results for: tet

1. pMSR:MSMEG_5447:Dendra

   Plasmid

   #167431

   Purpose

   C-term fluorescent fusion

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_5447
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-Lepr_Short-IRES-eGFP

   Plasmid

   #196935

   Purpose

   Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   short isoform, lack of signaling casacade

   Promoter

   TRE

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSR:MSMEG_0310:Dendra

   Plasmid

   #159051

   Purpose

   T7 expression of C-term fluorescent fusion in E. coli

   Depositor

   Joseph Wade, Keith Derbyshire

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0310
   

   Use

   Integrating mycobacterial expression vector attl5

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-PKHD1-myc

   Plasmid

   #108407

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mIFP12-CD81-10

   Plasmid

   #56248

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pcDNA5-GFP-PKHD1

   Plasmid

   #108408

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINTO-NFH::mEzh2

   Plasmid

   #65925

   Purpose

   Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   Enhancer of zeste homolog 2 (Ezh2 Mouse)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO (T-REx) and CMV/TetO2 (T-REx)

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-TSC22D3

   Plasmid

   #192916

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TSC22D3 (TSC22D3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid

    #157715

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-EF1_Cre

    Plasmid

    #201198

    Purpose

    AAV vector for cre expression under EF1 promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    cre
    EF1alpha promoter
    

    Use

    AAV

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    nuclear targeting cre

    Promoter

    N/A and hEF1alpha promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-CMV-Grid2(I677C)

    Plasmid

    #164782

    Purpose

    cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

    Insert

    Grid2 I677C (Grid2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I677C

    Promoter

    CMV

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gal4-entry+NLS

    Plasmid

    #128013

    Purpose

    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4 DBD-SV40NLS-3x-Flag
    

    Use

    Gal4 entry vector with nls to generate tethering …

    Tags

    Gal4-DBD-SV40NLS-3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid

    #205021

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT-mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINTO-NSA::mEzh2

    Plasmid

    #61725

    Purpose

    Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    Enhancer of zeste homolog 2 (Ezh2 Mouse)
    

    Use

    Tags

    mSA (monomeric streptavidin)

    Expression

    Mammalian

    Mutation

    Promoter

    T-REx (CMV + 2xTetO2)

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSR:MSMEG_0030:Dendra

    Plasmid

    #159040

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0030
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSR:MSMEG_5048:Dendra

    Plasmid

    #167366

    Purpose

    C-term fluorescent fusion

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5048
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge