We narrowed to 9,220 results for: Pol

1. pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

   Plasmid

   #174026

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-QF2
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Flag-Polß(L301R/V303R)-IRES-neo

   Plasmid

   #128664

   Purpose

   mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R

   Promoter

   CMV

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

   Plasmid

   #162936

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

   Plasmid

   #162937

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

   Plasmid

   #162935

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Maltose-binding protein fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Flag-Polß(L301R/V303R)-IRES-puro

   Plasmid

   #128658

   Purpose

   mammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

   Plasmid

   #162932

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion with AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Flag-Polß(L301R/V306R)-IRES-puro

   Plasmid

   #128656

   Purpose

   mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V306R

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Flag-Polß(K206A/K244A)-IRES-puro

    Plasmid

    #128683

    Purpose

    mammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K206A/K244A

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Flag-Polß(TM/K206A/K244A)-IRES-puro

    Plasmid

    #128684

    Purpose

    mammalian expression of Flag-Polß(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V303R/K206A/K244A

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

    Plasmid

    #176271

    Purpose

    Expression of CasMINI-V3.1 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI-V3.1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

    Plasmid

    #107313

    Purpose

    Encoding aCamkII-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

    Plasmid

    #177576

    Purpose

    Expresses SOUL in neuronal cell types

    Depositor

    Guoping Feng

    Article

    Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

    Insert

    SOUL
    

    Use

    AAV

    Tags

    tdTomato

    Promoter

    hSyn

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107311

    Purpose

    Encoding scFv-p65-HSF1 driven by EF1α promoter

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid

    #208961

    Purpose

    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

    Plasmid

    #174030

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-Venus-PEST
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

    Plasmid

    #48307

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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