We narrowed to 8,922 results for: Pol

1. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

   Plasmid

   #162933

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

   Plasmid

   #128657

   Purpose

   mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L301R/V303R/V306R

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

   Plasmid

   #172911

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon A122D
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

   Plasmid

   #166693

   Purpose

   Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9-VPR
   3x Cnga1 promoter-targeting sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and U6

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

   Plasmid

   #48294

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

   Plasmid

   #48308

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

    Plasmid

    #48284

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

    Plasmid

    #172912

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

    Plasmid

    #163084

    Purpose

    rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

    Depositor

    Lacra Bintu

    Article

    Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET StrepII TEV expression vector with BioBrick polypromoter restriction sites (14-R)

    Plasmid

    #48312

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

    Plasmid

    #48288

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET Mocr TEV expression vector with BioBrick polypromoter restriction sites (14-W)

    Plasmid

    #48315

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Mocr

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid

    #176139

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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