We narrowed to 2,400 results for: T2A

1. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-cGAS g3 Cas9-T2A-mCherry

   Plasmid

   #164254

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-T2A-Gal80-3xP3-RFP

   Plasmid

   #127562

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal80
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-EGFP-T2A-ACREB-WPRE

   Plasmid

   #40867

   Depositor

   Harold Gainer

   Article

   Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

   Insert

   ACREB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV hSyn1 CaRhGC wt T2A tDimer

   Plasmid

   #101720

   Purpose

   humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhGC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1 and ribosomal skip sequence T2A

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CAG-NCreIntN-T2A-mTagBFP2

   Plasmid

   #160500

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1a-mTagBFP-T2A-TetOn3G

   Plasmid

   #179888

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   mTagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS2CP(V29I)-myc-T2A-tagRFP

    Plasmid

    #140260

    Purpose

    Expresses MS2CP(V29I) in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Coat protein of bacteriphage MS2
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-T2A-LexGAD-3xP3-RFP

    Plasmid

    #127558

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    LexGAD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

    Plasmid

    #127564

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4DBD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-mCD59-T2A-GFP

    Plasmid

    #205451

    Purpose

    lentiviral plasmid for expression of mouse CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD59 (Cd59a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.U6.gDNMT1.Cas9-T2A-GFP

    Plasmid

    #170362

    Purpose

    Plasmid used for Crispr/cas9 based disruption of human DNMT1.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-T2A-p65-3xP3-RFP

    Plasmid

    #127565

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170324

    Purpose

    Expresses V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. XClone-Cas9-Cronus-T2A-BFP

    Plasmid

    #141209

    Purpose

    Tunable and temporal expression control of Cas9

    Depositor

    Rory Johnson

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. P-rTetO-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170325

    Purpose

    Tetracycline-inducible expression of V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-TREX1 g2 Cas9-T2A-mCherry

    Plasmid

    #164251

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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