We narrowed to 2,475 results for: T2A

1. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-moxGFP-P2A-T2A-Puro

   Plasmid

   #176893

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A
   

   Use

   Transposon

   Tags

   moxGFP

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-AU1

   Plasmid

   #162107

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-HA-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-HA-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG

   Plasmid

   #162112

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-HA-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-HA-FLAG

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA

   Plasmid

   #162089

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-HA
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-HA

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA

   Plasmid

   #162080

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-HA
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-HA

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

   Plasmid

   #62902

   Purpose

   Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4DBD-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pdgfra-T2A-H2B-eGFP donor

   Plasmid

   #113120

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

   Plasmid

   #62957

   Purpose

   Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4 in three phases
   

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHACK(Gal4)-DONR(T2A-Flp1)

    Plasmid

    #194770

    Purpose

    To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-Flp1
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160502

    Purpose

    Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-T2A-FLPo-3xP3-RFP

    Plasmid

    #127561

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    FLPo
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-EGFP-T2A-ACREB-WPRE

    Plasmid

    #40867

    Depositor

    Harold Gainer

    Article

    Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

    Insert

    ACREB
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. P-Ef1a-EGFP-T2A-RPL22-1xFlag

    Plasmid

    #170323

    Purpose

    Expresses FLAG and EGFP tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV hSyn1 CaRhGC wt T2A tDimer

    Plasmid

    #101720

    Purpose

    humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhGC
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1 and ribosomal skip sequence T2A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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