We narrowed to 14,582 results for: eng
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Plasmid#168189PurposeHA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nUbc6E-(EAAAK)4-OGT(4,K852A)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
Plasmid#159863PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159861PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159867PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS4 (Empty insertion vector with SEC)
Plasmid#154837PurposeVector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selectionDepositorInsertSEC
UseCRISPR and Cre/LoxExpressionWormMutationsqt-1(e1350)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (Merlin)
Plasmid#136448PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-lengthDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertglycoprotein H (UL75 Human betaherpesvirus 5)
ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (Merlin)
Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
TagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2b-srt-his
Plasmid#124804PurposeHDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMurine IgG2b
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
ExpressionMammalianPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-E6I4Q5
Plasmid#103101PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
Plasmid#107163PurposeExpresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzymeDepositorInsertF37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
TagsGSTExpressionBacterialPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain o…Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX398 Nitratifractor salsuginis str DSM 16511 Cas9
Plasmid#68334PurposeNitratifractor salsuginis str DSM 16511 Cas9DepositorInsertNsCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-Keratin14-N-18
Plasmid#56346PurposeLocalization: Cytokeratin, Excitation: 530, Emission: 541DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKeratin14 (KRT14 Human)
TagsmPapayaExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-LaminA-C-18
Plasmid#56397PurposeLocalization: Nuclear Envelope, Excitation: 530, Emission: 541DepositorAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-tetO-Mek1-shRNA
Plasmid#53179PurposeExpresses shRNA against mouse Mek1 from puromycin resistance retroviral vectorDepositorAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx80
Plasmid#36945DepositorInsertTrx80 (truncated Trx) (TXN Human)
TagsHis tagExpressionBacterialMutationpremature stop inserted after K80 in Trx1 genePromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
CACNA1D (2of2) or cav1.3b_L (OZ547)
Plasmid#28072DepositorInsertZinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)
UseZebrafish targetingAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only