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pG418R-MCS-LexA-VP16-MiniWhite

Plasmid

#165894

Purpose

G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HypocratesCS

Plasmid

#161957

Purpose

Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

Depositor

Dmitry Bilan

Article

Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

Insert

HypocratesCS

Tags

6xHIs tag

Expression

Bacterial

Mutation

changed cysteine 355 to serine

Promoter

T5

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-mCherry-GMRWhite

Plasmid

#165908

Purpose

Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-GMRWhite

Plasmid

#165920

Purpose

Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-sfGFP-MiniWhite

Plasmid

#165916

Purpose

Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-MiniWhite

Plasmid

#165923

Purpose

Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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DT033A

Plasmid

#159394

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer-NR2F2-MUT

Plasmid

#138371

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161k human enhancer with conserved NR2F2 binding sites mutated

Use

Gateway compatible

Mutation

NR2F2 sites mutated

Promoter

none

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer-ETS-CORE-MUT

Plasmid

#138372

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

human 161K human enhancer with ETS bind sites mutated

Use

Gateway compatible

Mutation

ETS factor binding sites mutated

Promoter

NONE

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

Plasmid

#114372

Purpose

expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Nlgn1C (NEWENTRY )

Use

AAV

Tags

EYFP and Nlgn1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMElmo2P1gfp

Plasmid

#90127

Purpose

Gateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edll4E1

Plasmid

#90120

Purpose

5' Gateway entry clone containing endothelial specific enhancer 1 sequence of dll4

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E1 (dll4 Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Elmo2E1

Plasmid

#90118

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of lmo2

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2E1 (lmo2 Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edusp5E1

Plasmid

#90122

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of dusp5

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1 (dusp5 Zebrafish)

Expression

Bacterial

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdusp5P1gfp

Plasmid

#90126

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dusp5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdll4P1gfp

Plasmid

#90125

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dll4 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edll4E2

Plasmid

#90121

Purpose

5' Gateway entry clone containing endothelial specific enhancer 2 sequence of dll4

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E2 (dll4 Zebrafish)

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

Plasmid

#69231

Purpose

Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 TALE

Use

CRISPR

Tags

2X NLS and NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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