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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGS1222

Plasmid

#63791

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

eGFP-Twin-Strep tag followed by URA3

Use

E. coli cloning vector

Tags

eGFP-Twin-Strep tag

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T-HEPN

Plasmid

#75296

Purpose

Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

Insert

Human Sacsin 4441-4579

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6020nes

Plasmid

#108656

Purpose

A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-JNK-EV

Tags

XFP

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-mAV

Plasmid

#129472

Purpose

Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

Depositor

Elizabeth Luna

Article

Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

Insert

Supervillin, isoform 2 (Svil Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

Plasmid

#123189

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Expression

Mammalian

Promoter

t7

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_H2B_MS2

Plasmid

#81082

Purpose

Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_H2B_24xMS2

Expression

Mammalian

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gβγ-BERKY1

Plasmid

#158429

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK3ct

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
C1-MPAct(delta1-6)-mRuby3

Plasmid

#155223

Purpose

F-actin bidning mutant of MPAct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-Flag-mMcl-1 KR

Plasmid

#32983

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pAd-PV-NLS-CD-DsRed

Plasmid

#16345

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS-CD (Pvalb Rat)

Use

Adenoviral

Tags

Myc

Expression

Mammalian

Mutation

Functional Ca2+binding site CD was inactivated by…

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Tags

flag

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-LPETGG

Plasmid

#91697

Purpose

CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

CohIII-CBM-HIS-LPETGG

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only