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pNatA (pACYCduet-naa10-naa15)

Plasmid

#72928

Purpose

Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

naa10 (naa10 Synthetic, Fission Yeast)
naa15 (naa15 Fission Yeast)

Expression

Bacterial

Mutation

Codon optomised for E.coli expression

Promoter

T7

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ST-SBP-EGFP

Plasmid

#65267

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST LIN28A

Plasmid

#43797

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28A (LIN28A Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-14-3-3 Beta

Plasmid

#19957

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

14-3-3 Protein Beta (YWHAB Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRS40B

Plasmid

#35478

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE1a-RFP

Plasmid

#35331

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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Human HOXA9-MSCV-short

Plasmid

#20978

Depositor

Corey Largman

Article

Vijapurkar et al Mol Cell Biol. 2004 May . 24(9):3827-37.

Insert

HOXA9 (HOXA9 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Missing amino acid #1. Missing 3'UTR. Coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pBABE-puro SHP2 D61A

Plasmid

#8330

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D61A (activated mutant)

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
SIVu

Plasmid

#81089

Purpose

Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMA3211

Plasmid

#46879

Purpose

Lentiviral vector for Tet-inducible expression. Puromycin-resistance

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Type

Empty backbone

Use

Lentiviral

Promoter

TRE-Tight

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRSII316

Plasmid

#35455

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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eNOS S1179A

Plasmid

#22485

Depositor

William Sessa

Article

Fulton et al Nature. 1999 Jun 10. 399(6736):597-601.

Insert

eNOS S1179A

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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