We narrowed to 83,854 results for: TRI

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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPTapp-Kras

Plasmid

#235999

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPTapp-Kras (PLEKHA1 Synthetic, Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Pancortin scFv [K96/7]

Plasmid

#190497

Purpose

Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.Cas9.P2A.BSD

Plasmid

#181981

Purpose

Lentiviral Cas9 expression

Depositor

Jan-Henning Klusmann

Article

Gialesaki et al Blood. 2023 Mar 9;141(10):1105-1118. doi

Insert

Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))

Use

CRISPR and Lentiviral

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-iC++-EYFP

Plasmid

#137155

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon/Von eYFP

Plasmid

#137164

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV Retrograde and AAV8

Insert

3x-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

Plasmid

#166010

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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TrpC5 scFv [N67/15]

Plasmid

#190564

Purpose

Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PA-DAD-wt

Plasmid

#53095

Purpose

Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

LOV(wt)-6aa-DAD

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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SPHKAP scFv [L131/17]

Plasmid

#190511

Purpose

Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA1

Plasmid

#201590

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pan-Synapsin scFv [L125/129]

Plasmid

#182079

Purpose

Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting pan-Synapsin

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#190504

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

Plasmid

#166011

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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BII-BCA-Prom-ASCL2

Plasmid

#133394

Purpose

Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

destabilized tdTomato-NLS and destabilized NLS-GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free) drives expressi…

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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