We narrowed to 31,176 results for: LIS

1. PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

   Plasmid

   #170827

   Purpose

   PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d ANXA2 gRNA2
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

   Plasmid

   #170828

   Purpose

   PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d CLCN3 gRNA1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

   Plasmid

   #170829

   Purpose

   PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d CLCN3 gRNA2
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

   Plasmid

   #170822

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAM-AAV-hIsl2-EGFP

   Plasmid

   #153206

   Purpose

   Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-TEX264wt-cFlag

   Plasmid

   #220204

   Purpose

   Mammalian expression of wild type TEX264, C-terminal Flag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-TEX264-E194A-cFlag

   Plasmid

   #220207

   Purpose

   Mammalian expression of TEX264 with E194A mutation, C-terminal Flag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   194E>A

   Promoter

   CMV

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

    Plasmid

    #207025

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A D384A Y387A

    Promoter

    pTEF1

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCUP1-GFP-Pho8-406

    Plasmid

    #207006

    Purpose

    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

    Plasmid

    #217509

    Purpose

    KD Recombinase dependent conditional 1X ALFA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
    

    Use

    Tags

    ALFA tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCUP1-GFP-Osh1-406

    Plasmid

    #207007

    Purpose

    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    OSH1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B2M-SDMutation-EhT

    Plasmid

    #215546

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    The second base of the first intron of B2M (From …

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. B2M-SDMutation-EPG

    Plasmid

    #215545

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    The second base of the first intron of B2M (From …

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET SUMO-Doc2β C2AB (G361C)

    Plasmid

    #213631

    Purpose

    Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    Doc2β C2AB (G361C) (Doc2b Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    G361C, all original cysteines mutated to alanines

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

    Plasmid

    #207050

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    P85L P86L

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

    Plasmid

    #207051

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    T56A R65A T87A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

    Plasmid

    #207052

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85A P86A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge