We narrowed to 19,027 results for: Tec

1. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. QQR1 variant

   Plasmid

   #138564

   Purpose

   Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   QQR1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_OT1.0

   Plasmid

   #185386

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

   Plasmid

   #138610

   Purpose

   Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

   Depositor

   Fei Chen

   Article

   Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   Hyperactive AID
   T7 RNA Polymerase
   tdTomato
   

   Expression

   Mammalian

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti Lifeact-iRFP670 BlastR

   Plasmid

   #84385

   Purpose

   Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   iRFP670

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLM-CMV-R-Cre

   Plasmid

   #27546

   Purpose

   Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

   Insert

   mCherry_P2A_Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   CMV

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEC3109 (pSpy1C-Plac(1)_ffluc)

   Plasmid

   #218514

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(1)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYFAC-CH2-ubi-Y-pyrG

    Plasmid

    #221131

    Purpose

    Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

    Type

    Empty backbone

    Use

    Synthetic Biology; Expression in aspergillus and …

    Promoter

    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115219

    Purpose

    Expresses PspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PspCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRSF-G1TFAKRS

    Plasmid

    #177311

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEmax_2star_IVT

    Plasmid

    #227674

    Purpose

    plasmid for PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5.sgRNA.EFS.tRFP

    Plasmid

    #57823

    Purpose

    3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. NO MS-Sensor (35)

    Plasmid

    #199783

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    NO MS-Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAF0432 Ef1a-Dn29-2a-EGFP

    Plasmid

    #193459

    Purpose

    Expression of Dn29 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9 TadCBEd

    Plasmid

    #193835

    Purpose

    Express TadCBEd (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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