We narrowed to 28,492 results for: tec

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pRDA_789

Plasmid

#216078

Purpose

CROPseq, CMV-driven destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral; Destination

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_512

Plasmid

#216075

Purpose

RSV-driven, lentiGuide-based destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_832

Plasmid

#216085

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA095

Plasmid

#216043

Purpose

Fragmid fragment: (C' terminus) ZNF10 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA050

Plasmid

#216059

Purpose

Fragmid fragment: (2A Selection) violet-excited GFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; VexGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_166

Plasmid

#216093

Purpose

Cas9 [Sp] CRISPRi targeting CD55, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD55 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA370

Plasmid

#215988

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-LB-geneticin

Plasmid

#213468

Purpose

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Left border of the TSI locus 1-split fragment of geneticin cassette

Expression

Bacterial

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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p4A8_M59I_T94M_BC

Plasmid

#217833

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Expression

Yeast

Mutation

VH: M59I, VL: T94M

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pNS49 (enAsCas12a-KRAB piggyBac)

Plasmid

#217335

Purpose

piggyBac expresion of enAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

enAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIDAF

Plasmid

#213708

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIBY55

Plasmid

#213707

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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2M-Mx-FLAG

Plasmid

#206942

Purpose

Mammalian Expression of 2M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.5

Plasmid

#208316

Purpose

Expresses ABE-RA5.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.5-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG

Plasmid

#206938

Purpose

Mammalian Expression of 1M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMXSIP-Rab5B-HA

Plasmid

#204709

Purpose

expresses Rab5B-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

RAB5B (RAB5B Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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3M-Qt-FLAG

Plasmid

#206940

Purpose

Mammalian Expression of 3M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, SeSmtA, TaEC1, QtEnc

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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2M-Qt-FLAG

Plasmid

#206939

Purpose

Mammalian Expression of 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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