We narrowed to 18,339 results for: Mut

1. pHHM.X513-iNicSnFR2-(CC90)

   Plasmid

   #124836

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR2-(CC90)
   

   Use

   Tags

   HA tags and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-deltaA1-R1597W-VWF

   Plasmid

   #124826

   Purpose

   Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF without A1 domain and R1597W mutation A2 domain (VWF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-deltaA1-D1498A-VWF

   Plasmid

   #124825

   Purpose

   Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF without A1 domain and D1498A mutation A2 domain (VWF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-001428(753)-VC

   Plasmid

   #123256

   Purpose

   Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (001428) Env
   

   Use

   Tags

   CD5 leader peptide and VC (Venus residues D155–K2…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; C-terminal Env re…

   Promoter

   CMV

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19_T790M_28bp

   Plasmid

   #114721

   Purpose

   28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   c.2369C>T (Substitution, position 2369, C➞T); …

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_PKA-Cα(S11D)-mPAGFP

   Plasmid

   #114135

   Purpose

   Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3_PKA-Cα(S11A)-mPAGFP

   Plasmid

   #114134

   Purpose

   Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19_4xT790M_28bp

   Plasmid

   #114719

   Purpose

   4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

   Depositor

   Nils Walter

   Article

   Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   c.2369C>T (Substitution, position 2369, C➞T); …

   Promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PAK4-FL-3'UTR-MT2

   Plasmid

   #113093

   Purpose

   Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated

   Depositor

   James Donahue

   Article

   Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

   Insert

   p21 Activated kinase 4 (PAK4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Second predicted miR-199a-3p binding site mutated…

   Promoter

   PGK

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PAK4-FL-3'UTR-MT-both

    Plasmid

    #113094

    Purpose

    Luciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutated

    Depositor

    James Donahue

    Article

    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

    Insert

    p21 Activated kinase 4 (PAK4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Both the predicted miR-199a-3p binding site muta…

    Promoter

    PGK

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiV_Neo_LKB1_CR

    Plasmid

    #108112

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change cytosine 333 to alanine (silent mutation)

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCh-607

    Plasmid

    #92258

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMCh-633

    Plasmid

    #92253

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUPI hT5Cyp-H126Q

    Plasmid

    #80281

    Purpose

    Expresses human TRIM5 CypA fusion with H126Q mutation in CypA

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion (TRIM5, PPIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H126Q mutation in CypA

    Promoter

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZM3[I:FruR-L,I:RbsR-L]

    Plasmid

    #60777

    Purpose

    Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    FruR-L
    RbsR-L
    

    Use

    Tags

    Expression

    Mutation

    LacI/GalR repressor chimera. LacI DBD with FruR L…

    Promoter

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2 mir-1-1 reporter mir16-1.3

    Plasmid

    #46682

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.3 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2 mir-1-1 reporter mir16-1.1

    Plasmid

    #46680

    Purpose

    Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1.1 (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET-20b-T7-Ntx-K49

    Plasmid

    #58715

    Purpose

    Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic Acid 49 to Lysine

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPC-Flag-CHES1H164A

    Plasmid

    #51844

    Purpose

    Flag-CHES1L mutant with ponctual mutation on histidine 164

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only