We narrowed to 41,611 results for: KAN

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289aa Met133, 138, 186Ileu ELL2

Plasmid

#127272

Purpose

Expresses 289aa human ELL2 with Met133, 138, 186Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)

Expression

Mammalian

Mutation

Met133, 138, 186 Ileu, cut with XbaI blunt fillin

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV29

Plasmid

#123401

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV29 (TRAV29DV5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-192-Reporter

Plasmid

#71872

Purpose

Mammalian expression vector for the analysis of miR-19α activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-192

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-193a-5p-Reporter

Plasmid

#71873

Purpose

Mammalian expression vector for the analysis of miR-193a-5p activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-193a-5p

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-31-192-193a-Reporter

Plasmid

#71874

Purpose

Mammalian expression vector for the analysis of miR-31-19α-193a activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Binding sequence targeted by miR-31, miR192, and miR-193a-5p

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestGFP_RBPMS2

Plasmid

#65747

Purpose

expresses GFP-tagged RBPMS2 in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

GFP

Expression

Mammalian

Mutation

S13N mutation in RBPMS2

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-n

Plasmid

#53077

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-cyt-n

Plasmid

#53065

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-er-n

Plasmid

#53067

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-n

Plasmid

#53071

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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MS1

Bacterial Strain

#61960

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-DIO-ATLASsnFlp

Plasmid

#232352

Purpose

Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Has Service

AAV5 and AAV8

Insert

ATLASsnFlp

Use

AAV

Tags

ALFA-Tag

Promoter

hSyn

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-BACE-HA

Plasmid

#232353

Purpose

Beta-Secretase to be expressed (1:1) with either ATLAS protein.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Has Service

AAV8

Insert

BACE (BACE1 Human)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pIND21-puro_SOX10-3xFLAG

Plasmid

#250322

Purpose

Tetracycline-inducible expression of SOX10. rtTA (Tet-ON) is driven by the EF1alpha promoter.

Depositor

Michael Wehr

Article

Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8-C403S

Plasmid

#221685

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

C403S

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8

Plasmid

#221683

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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mGLDC

Plasmid

#72881

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

Gldc (Gldc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only