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pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-Linker-C6-His

Plasmid

#233331

Purpose

To express nanobody C6 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-Linker-C6-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH16-His

Plasmid

#233332

Purpose

To express nanobody VHH16 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH16-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH21-His

Plasmid

#233333

Purpose

To express nanobody VHH21 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH21-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_1-His

Plasmid

#233334

Purpose

To express nanobody IGHJ4_1 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_1-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2618

Plasmid

#234497

Purpose

NEOR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Article

Nalley et al bioRxiv 2025.02.14.638277

Insert

NEO-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2616

Plasmid

#234495

Purpose

HYGR-CnU6-empty sgRNA

Depositor

Hiten Madhani

Article

Nalley et al bioRxiv 2025.02.14.638277

Insert

HYG-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVP084

Plasmid

#222036

Purpose

meffBlue chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

meffBlue

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP085

Plasmid

#222037

Purpose

spisPink chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

spisPink

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP086

Plasmid

#222038

Purpose

mRFP1e chromoprotein in a MultiGreen 1.0 HG entry vector.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV_CMV_cMyc-VSVG

Plasmid

#212644

Purpose

Lentivirus vector to express cMyc-VSVG

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

VSVG

Use

Lentiviral

Tags

cMyc tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-VSVG

Plasmid

#212646

Purpose

piggyBac vector to express FLAG-VSVG

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

VSVG

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_HA-tNGFR_ZeoR

Plasmid

#212650

Purpose

piggyBac vector to express HA-tNGFR with zeocin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_PuroR

Plasmid

#212651

Purpose

piggyBac vector to express FLAG-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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p32.pA.NGFR-mSrtA(Ge)-flag.mCMV.hPGK.GFP.wpre[sequenced]

Plasmid

#226729

Purpose

sortase A(GE) in p30

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

flag

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM81987

Plasmid

#219516

Purpose

Silencing of Nb RT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb RT

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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