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pDONR223_RHEB_WT

Plasmid

#81257

Purpose

Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHEB (RHEB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLT06

Plasmid

#255019

Purpose

Markerless deletion vector that enables manipulation of enterococcus to either insert DNA or delete DNA from the genome. Contains resistance gene for chloramphenicol resistance.

Depositor

Lynn Hancock

Article

Thurlow et al J Bacteriol. 2009 Oct;191(20):6203-10. d

Type

Empty backbone

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pEHAC1-CHIP-BirA-HA

Plasmid

#232587

Purpose

Express BirA tagged to the C-terminus of CHIP.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

CHIP - BirA (CHIP Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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CROPSeq-PP7-p65-HSF

Plasmid

#196719

Purpose

CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP670nano3-NbGFP

Plasmid

#184686

Purpose

Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-NbGFP

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_minus_cer-region

Plasmid

#175297

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Type

Empty backbone

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CTTN-1/1

Plasmid

#91550

Purpose

Protein expression and purification of human SH3 domain construct CTTN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CTTN-1/1 (CTTN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDXC3GMS

Plasmid

#49031

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRetro-CAG-DsRedExpress-G-TVA

Plasmid

#235698

Purpose

Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800, and Glyco

Use

Retroviral

Promoter

CAG

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas6

Plasmid

#170091

Purpose

expresses E. coli type I-E Cas6

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1513

Plasmid

#108998

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1515

Plasmid

#109000

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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