We narrowed to 30,841 results for: Ide

1. MCP-EGFP-PiggyBac

   Plasmid

   #190003

   Purpose

   Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

   Insert

   MCP-EGFP
   

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMetYC-Dest

   Plasmid

   #105081

   Purpose

   Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Tags

   Cub-PLV

   Expression

   Yeast

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACS129

   Plasmid

   #204594

   Purpose

   Histone H3.1-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 (H3C3 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1-V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTurquoise2-ER

   Plasmid

   #36204

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   ER-mTurquoise2
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hNET pcDNA3

   Plasmid

   #15475

   Depositor

   Randy Blakely

   Article

   Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

   Insert

   hNET (SLC6A2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYCDuet1-tglB

   Plasmid

   #134676

   Purpose

   Expresses His6-TglBin E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglB
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKL10-mRuby-iATPSnFR1.0 bb118

   Plasmid

   #158980

   Purpose

   Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   mruby-iATPSNfR1.0
   

   Tags

   mruby3

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#102557

   Promoter

   Bba_J23118 (sigma 70)

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Keratin 18 (pcDNA3)

   Plasmid

   #18064

   Depositor

   M Bishr Omary

   Article

   Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

   Insert

   Keratin 18 (KRT18 Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1B-GFP1-10

    Plasmid

    #193867

    Purpose

    E. coli expression vector for Hisx6 tagged GFP1-10.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    sfGFP1-10
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJExpress414_GmPOPB-H698N

    Plasmid

    #92242

    Purpose

    Express H698N mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698N mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Asparagine

    Promoter

    T7 promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2B -1538/+64

    Plasmid

    #24942

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Available Since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. MiniCoopR U6:gRNA, mitfa:Cas9

    Plasmid

    #118840

    Purpose

    Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-HxB.L

    Plasmid

    #65414

    Purpose

    contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    TNC large splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-)-Green Falcan0.3

    Plasmid

    #199704

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan0.3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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