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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,819 results for: OMP;

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Showing: 6961 - 6980 of 16819 results

  6. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188747
    Purpose
    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    UbC
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188747-plasmid-map-sequence-id-371444

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  7. NSP4-WT-Flag

    Plasmid
    #210339
    Purpose
    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Publication
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210339-plasmid-map-sequence-id-418188

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  8. pcDNA 3.1-nsp7-FLAG

    Plasmid
    #201027
    Purpose
    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.
    Depositor
    Sharon Rozovsky
    Publication
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    8xHis and FLAG
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201027-plasmid-map-sequence-id-394458

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  9. pD649-HAsp-HAVCR1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156712
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HAVCR1 (HAVCR1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156712-plasmid-map-sequence-id-426450

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  10. pD649-HAsp-TNFRSF1B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156686
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156686-plasmid-map-sequence-id-424369

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  11. his3MX6-ins3

    Plasmid
    #195040
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Publication
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    His5+
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195040-plasmid-map-sequence-id-384243

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  12. pD649-HAsp-EDAR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156554
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    EDAR (EDAR Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156554-plasmid-map-sequence-id-368528

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  13. pAc5.1B-DmPABPC1-V5His6_D

    Plasmid
    #146093
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Expression
    Insect
    Mutation
    one non-silent mutation G26S compared to the sequ…
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146093-plasmid-map-sequence-id-362948

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  14. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  15. Epac-S-H81

    Plasmid
    #170339
    Purpose
    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170339-plasmid-map-sequence-id-344640

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  17. Epac-S-H90

    Plasmid
    #170340
    Purpose
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170340-plasmid-map-sequence-id-335919

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  19. Venus1-K49R-STAT3

    Plasmid
    #123166
    Purpose
    Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    K49R substitution
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123166-plasmid-map-sequence-id-238632

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  20. Venus2-K49R-STAT3

    Plasmid
    #123167
    Purpose
    Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    K49R substitution
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123167-plasmid-map-sequence-id-238656

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Showing: 6961 - 6980 of 16819 results