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pGL0_115 [pBC1 ORI]

Plasmid

#199015

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2-Synapsin1a

Plasmid

#191567

Purpose

Expresses an shRNA and mTagBFP2-synapsin1a

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

Plasmid

#167899

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

Plasmid

#167897

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pAF4E9D

Plasmid

#185844

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>LacI>TUP1>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9C3

Plasmid

#185843

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>RevTetR_r1.7>SIN3C>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9D91

Plasmid

#185845

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>SIN3C>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAFZifPZ432

Plasmid

#185850

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED15>PAFB1

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9D93

Plasmid

#185847

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>MIG1C>PABF1

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9D92

Plasmid

#185846

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>TUP1>PABF1

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAF7E1

Plasmid

#185839

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>MED15>PABF1

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAF4EH4R

Plasmid

#185837

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>Zif268>VP16-PABF1

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK036

Plasmid

#180828

Purpose

T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmA

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

Plasmid

#167896

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC034

Plasmid

#171153

Purpose

pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

J3(106)- BBa_J23111-sfGFP

Expression

Bacterial

Promoter

J3(106)-BBa_J23111

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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UBQLN2_Halo_C_allele

Plasmid

#178119

Purpose

Donor vector for endogenous tagging of human UBQLN2 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (UBQLN2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUEEN BPKK

Plasmid

#129381

Purpose

mutant of QUEEN 84BP

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN BPKK

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET QUEEN7mu R103A

Plasmid

#129372

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN7mu R103A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET QUEEN7mu K114A

Plasmid

#129373

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN7mu K114A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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