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pHR-flag-GFP-DLL1

Plasmid

#205192

Purpose

This vector encodes of a synCAM (DLL1) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM DLL1 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-MUC4

Plasmid

#205189

Purpose

This vector encodes of a synCAM (MUC4) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM MUC4 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-NLS-BirA-2A-mCherry

Plasmid

#127781

Purpose

BirA construct in a PCI nls backbone driving mCherry activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

BirA

Use

Unspecified

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYUU

Plasmid

#159751

Purpose

Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR L1-5XQUAS-R5

Plasmid

#32303

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

5XQUAS

Use

Gateway multisite entry vector

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR L5-GAL4-L2

Plasmid

#32304

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

GAL4 (GAL4 Budding Yeast)

Use

Gateway multisite entry clone

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

Plasmid

#209081

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T, NAC deletion (aa60-95)

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

Plasmid

#197067

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

MitoTrap-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-NCAM-1

Plasmid

#205199

Purpose

This vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM NCAM 1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCJ111

Plasmid

#175052

Purpose

rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJ2048.2

Plasmid

#140687

Purpose

3-color stripe (different sgRNAs)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (different sgRNAs)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

Plasmid

#85438

Purpose

Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

S. typhi FliC

Use

Lentiviral

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10871

Plasmid

#183962

Purpose

U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

dCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A

Promoter

CAG and U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAKgfplux3

Plasmid

#199304

Purpose

Fluorescence and bioluminescence expression in Gram-negative bacteria

Depositor

Attila Karsi

Article

Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

Insert

Chloramphenicol acetyltransferase

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ11353

Plasmid

#183098

Purpose

pHR-CAG-sfGFP* ABE_3-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

GFP*

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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