We narrowed to 10,322 results for: Coli
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Plasmid#248920PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfGFP for nanodisc formationDepositorInsertMSP1D1_sfGFP
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1_mCherry
Plasmid#248922PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mCherry for nanodisc formationDepositorInsertMSP1D1_mCherry
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1_mBlueberry2
Plasmid#248925PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mBlueberry2 for nanodisc formationDepositorInsertMSP1D1_mBlueberry2
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKP2009
Plasmid#167602PurposeExpress ExbB (215-224 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 215-22…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2010
Plasmid#167603PurposeExpress ExbB (235-244 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 235-24…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2019
Plasmid#167604PurposeExpress ExbB (195-204 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 195-20…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2040
Plasmid#167650PurposeExpress His6-ExbB S80A, S82A, S84ADepositorInserthis6-exbB S80A, S82A, S84A
ExpressionBacterialMutationExbB S80A, S82A, S84A with His6 tag at N terminusAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2025
Plasmid#167605PurposeExpress ExbB (225-234 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 225-23…Available SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2008
Plasmid#167601PurposeExpress ExbB (205-214 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 205-21…Available SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
Tags6xHis, E-tag, and myc-tagExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
ExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)
Plasmid#231130PurposeExpresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.DepositorInsertE. coli tyrosyl tRNA synthetase
ExpressionBacterialMutationEcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…Available SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR(NGS-6)-mCherry
Plasmid#217363PurposeExpresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA mutant, "NGS-6"
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTET-IUPi
Plasmid#122019PurposeExpresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpTETAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N+C-term K-R
Plasmid#233093PurposeExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with N+C-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with N+C-term lysines (K) mutated to arg…Available SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term K-R
Plasmid#233091PurposeExpression of GST-YihI with N-term lysines (K) mutated to arginine (R)DepositorInsertYihI with N-term lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationN-terminal lysine residues mutated to arginineAvailable SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-All K-R
Plasmid#233094PurposeExpression of GST-YihI with all lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with all lysines (K) mutated to arginine (R)
TagsGSTExpressionBacterialMutationGST-YihI with all lysines (K) mutated to arginine…Available SinceMay 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRAR051
Plasmid#185029Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-51 & 80-84 mutated rpmE 5'…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR052
Plasmid#185030Purpose400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 56-61 and 68-73 mutated in rpmE 5…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR104
Plasmid#185034Purpose400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationA74 and A76 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR105
Plasmid#185035Purpose400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG52 and G79 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR107
Plasmid#185037Purpose400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationAGA74-76GAG mutation of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR109
Plasmid#185038Purpose400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG60 and C69 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR110
Plasmid#185039Purpose400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG62 and G67 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR039
Plasmid#185025Purpose400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 1-31 deleted from rpmE 5'UTR, On…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR040
Plasmid#185026Purpose400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 35-46 deleted from rpmE 5'UTR, O…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR041
Plasmid#185027Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-54 & 76-86 deleted from rpmE …Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCapVQ329R
Plasmid#183029Purposethe patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28DepositorInsertpatatin-like phospholipase variant CapVQ329R
TagsNoExpressionBacterialMutationglutamine 329 changed to argininePromoterpBAD promoterAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ec-iPGM-bio
Plasmid#162575PurposeExpresses E. coli iPGM containing a C-terminal biotinylation site and His tagDepositorInsertE. coli iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP019
Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
ExpressionBacterialMutationH134R mutationPromoteraraBADAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hygro-CMV-TetO2__HA-BirA-ER
Plasmid#249352PurposeMammalian (inducible) in vivo biotinylationDepositorInsertBirA-ER
UseLentiviralPromoterCMV-MIEAvailable SinceMarch 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHR-Blast-CMV-TetO2__HA-BirA-ER
Plasmid#249353PurposeMammalian (inducible) in vivo biotinylationDepositorInsertBirA-ER
UseLentiviralPromoterCMV-MIEAvailable SinceMarch 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1_sfCherryL
Plasmid#248924PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfCherry for nanodisc formation (longer linker)DepositorInsertMSP1D1_sfCherryL
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 6, 2026AvailabilityIndustry, Academic Institutions, and Nonprofits