We narrowed to 3,255 results for: GAI

1. pRZ-mNanog-YNL

   Plasmid

   #65717

   Purpose

   mNanog reporter plasmid (expresses YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   murine Nanog promoter and YNL
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-6017nes

   Plasmid

   #108655

   Purpose

   A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-PKA-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT/FLAG/HA-DEST EIF2C4

   Plasmid

   #19890

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Eukaryotic translation initiation factor 2C, 4 (AGO4 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-6015nes

   Plasmid

   #108657

   Purpose

   A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-S6K-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSIIbsr-6085x

   Plasmid

   #108653

   Purpose

   A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   PicchuEV
   

   Use

   Lentiviral

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETSUMO-TtAgocatdead

   Plasmid

   #210322

   Purpose

   Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

   Depositor

   Phillip Zamore

   Article

   Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

   Insert

   TtAgo (TT_P0026 Thermus thermophilus)
   

   Use

   Tags

   6x His tag and SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTorPE-B-GECO1

   Plasmid

   #32469

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   B-GECO1.0
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLTR-FP-GFP

   Plasmid

   #58242

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   attR1-Cm(R)-CcdB-attR2
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYNL-N1_TUBB5

   Plasmid

   #65678

   Purpose

   Expresses beta-tubulin-YNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   beta-tubulin class V
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Myc-Foxo1-WT 6KQ

    Plasmid

    #17559

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KQ (Foxo1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pBXNH3LCA_TM287/288

    Plasmid

    #132701

    Purpose

    expresses TM287/288 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    TM287/288 (TM0288, TM0287 Thermotoga maritima)
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-sg-tdTomato90/816

    Plasmid

    #179918

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdEos-Tubulin-C-18

    Plasmid

    #57683

    Purpose

    Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pJBL704

    Plasmid

    #140374

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-4BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-Teton-puro-shFAK #1

    Plasmid

    #198757

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #1 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1-Teton-puro-shFAK #2

    Plasmid

    #198758

    Purpose

    conditional knockdown of FAK

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

    Insert

    shFAK #2 (PTK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-RbE2Fmut

    Plasmid

    #32391

    Depositor

    Julien Sage

    Article

    Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

    Insert

    Rb promoter (Rb1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated E2F binding site: TTTCCCGC --> TTTACATC

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL705

    Plasmid

    #140375

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-5BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge