We narrowed to 4,855 results for: U6...

1. pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn

   Plasmid

   #159488

   Purpose

   Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn

   Plasmid

   #159482

   Purpose

   Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn

   Plasmid

   #159483

   Purpose

   Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn

   Plasmid

   #159484

   Purpose

   Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(6)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

   Plasmid

   #159485

   Purpose

   Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(7)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

   Plasmid

   #159486

   Purpose

   Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-1_PolyU(8)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

   Plasmid

   #81239

   Purpose

   U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

   Plasmid

   #117407

   Purpose

   NmeCas9 sgRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   NmeCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA

   Plasmid

   #117408

   Purpose

   CjeCas9 sgRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   CjeCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

    Plasmid

    #117409

    Purpose

    SauCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SauCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

    Plasmid

    #117410

    Purpose

    LbCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    LbCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128345

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb4 (Chrnb4 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128341

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna6 (Chrna6 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

    Plasmid

    #117137

    Purpose

    Expression vector encoding BFP-BSD-Nkx2-5

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

    Plasmid

    #84253

    Purpose

    Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

    Plasmid

    #84252

    Purpose

    Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

    Plasmid

    #190898

    Purpose

    Entry vector for multiple sgRNA cloning

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6 and 7sk

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

    Plasmid

    #129405

    Purpose

    circular Pepper RNA

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-Pepper(TAR Variant-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge