We narrowed to 12,543 results for: cel.2

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pLKO.1-Teton-puro-shPP1C #2

Plasmid

#198763

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #2 (PPP1CC Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L-A131L-T132L

Plasmid

#220304

Purpose

mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L-A131L-T132L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-SARS-CoV-2

Plasmid

#236423

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-30a-NSP10-SARS-CoV-2

Plasmid

#236426

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 N_D3L

Plasmid

#194806

Purpose

Sars-CoV-2 N_D3L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_N_D3L (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

N_D3L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_D63G

Plasmid

#194807

Purpose

Sars-CoV-2 N_D63G in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_D63G (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

D63G

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_R203M

Plasmid

#194810

Purpose

Sars-CoV-2 N_R203M in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203M (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

R203M

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_S235F

Plasmid

#194811

Purpose

Sars-CoV-2 N_S235F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_S235F (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

S235F

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-U6gRNA5(ZNF398_5-2)-PGKpuroBFP-W

Plasmid

#211998

Purpose

Express gRNA against ZNF398 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF398 (ZNF398 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

Plasmid

#211955

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 S_T1027I

Plasmid

#194850

Purpose

Sars-CoV-2 S_T1027I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_T1027I (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T1027I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_Y144del

Plasmid

#194854

Purpose

Sars-CoV-2 S_Y144del in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_Y144del (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

Y144del

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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