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BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 GamS

Bacterial Strain

#176584

Purpose

E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecB GamS

Bacterial Strain

#176585

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
AiP14573: pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA (Alias: CN4573)

Plasmid

#230871

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

Plasmid

#230880

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)

Plasmid

#214855

Purpose

Expression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

FlpO

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15747: pAAV-AiE0779m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5747)

Plasmid

#230882

Purpose

AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15649: pAAV-AiE0780m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5649)

Plasmid

#230879

Purpose

AiE0780m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13985: pAAV-AiE0445h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN3985)

Plasmid

#230865

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12909: pAAV-AiE0445h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2909)

Plasmid

#230862

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14646: pAAV-AiE0779m_6xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4646)

Plasmid

#230872

Purpose

AiE0779m_6xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14133: pAAV-AiE0445h_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4133)

Plasmid

#230867

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15582: pAAV-AiE0780m-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN5582)

Plasmid

#230878

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP15746: pAAV-AiE0780m-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5746)

Plasmid

#230881

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet

Use

AAV

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHAHA

Plasmid

#16586

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Type

Empty backbone

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pLIA (CC#1)

Plasmid

#15129

Depositor

Connie Cepko

Article

Bao et al J Neurosci. 1997 Feb 15. 17(4):1425-34.

Type

Empty backbone

Use

Retroviral

Tags

IRES-PLAP

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pBI-MCS-EGFP

Plasmid

#16542

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Type

Empty backbone

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pEBB modified

Plasmid

#22226

Depositor

David Baltimore

Article

Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 2, 2009

Availability

Academic Institutions and Nonprofits only

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7TP

Plasmid

#24309

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-PuroR

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pRBUBR1empty

Plasmid

#24505

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Type

Empty backbone

Expression

Yeast

Available Since

April 8, 2010

Availability

Academic Institutions and Nonprofits only