We narrowed to 3,496 results for: ubc

1. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

   Plasmid

   #225955

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Truncated CKAP4 (1-131) lacking the luminal domain

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

   Plasmid

   #225956

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Full-length CKAP4 with additional luminal domain …

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

   Plasmid

   #225939

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   STIM1 (STIM1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

   Plasmid

   #225940

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   STIM2 (STIM2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

   Plasmid

   #225944

   Purpose

   Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   ATL1 (ATL1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #218637

   Purpose

   Proximity biotinylation control of OSBP(PH domain)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   PH domain only

   Promoter

   UBC

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215012

   Purpose

   Express a ERphagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

    Plasmid

    #184556

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid

    #201186

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

    Plasmid

    #184557

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid

    #201187

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

    Plasmid

    #201188

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 3 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

    Plasmid

    #199447

    Purpose

    Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    dSaCas9-10xGCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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