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Sticky-nonpalindromic-169an-4X

Plasmid

#165413

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-167a-16X

Plasmid

#164494

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-169a-16X

Plasmid

#164495

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0173

Plasmid

#154640

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s (ΔAP2+1) Firefly luciferase expression cassette [35s (ΔAP2+1)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFVGe2

Plasmid

#149500

Purpose

Broad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, GmR, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-GmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLlCa5

Plasmid

#149501

Purpose

Broad-Host range expression vector, pBBR origin, LacI repressor, PLlacO-1 promoter, AmpR, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-AmpR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKPcR5

Plasmid

#149467

Purpose

Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

PcaU(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

P3B5B

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKVR5

Plasmid

#149469

Purpose

Broad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0258

Plasmid

#162291

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0025

Plasmid

#162283

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0512

Plasmid

#154751

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_057 Firefly luciferase expression cassette [MinSyn_057::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0513

Plasmid

#154752

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_062 Firefly luciferase expression cassette [MinSyn_062::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0483

Plasmid

#154730

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_115 Firefly luciferase expression cassette [MinSyn_115::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0484

Plasmid

#154731

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_116 Firefly luciferase expression cassette [MinSyn_116::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0485

Plasmid

#154732

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_117 Firefly luciferase expression cassette [MinSyn_117::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0486

Plasmid

#154733

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_118 Firefly luciferase expression cassette [MinSyn_118::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0487

Plasmid

#154734

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_119 Firefly luciferase expression cassette [MinSyn_119::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0489

Plasmid

#154736

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_121 Firefly luciferase expression cassette [MinSyn_121::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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