We narrowed to 14,099 results for: cas9

1. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-LoxP-GFP-LoxP-SNAP-TERT

   Plasmid

   #71391

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRPM7 gRNA (BRDN0001147821)

   Plasmid

   #76112

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRPM7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRPM7 (TRPM7 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL035

   Plasmid

   #137903

   Purpose

   STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAT-PB-CG2APtk

   Plasmid

   #86003

   Purpose

   homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   eGFP_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIK3C2B gRNA (BRDN0001149054)

   Plasmid

   #76712

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIK3C2B gRNA (BRDN0001149112)

   Plasmid

   #76713

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEL063

   Plasmid

   #124895

   Purpose

   For genome editing in plant by SpCas9 variant(SpCas9-NG)

   Depositor

   Yong Zhang

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEL066

   Plasmid

   #124897

   Purpose

   For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

   Depositor

   Yong Zhang

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYLsgRNA-OsU6a/LacZ

    Plasmid

    #66195

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU6a

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-CADPS KI

    Plasmid

    #131482

    Purpose

    Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cadps Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1163

    Plasmid

    #111099

    Purpose

    Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCD2 gRNA (FANCD2 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKD1 gRNA (BRDN0001148399)

    Plasmid

    #76842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TNIK gRNA (BRDN0001146045)

    Plasmid

    #75849

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. gh141

    Plasmid

    #106811

    Purpose

    expression of gRNA targeting OGT

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    OGT (OGT Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEL031

    Plasmid

    #137900

    Purpose

    STU2.0 SpyCas9 tRNA system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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