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p3xFLAG-CMV-Tlr4(Lps)

Plasmid

#27149

Depositor

Bruce Beutler

Publication

Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

toll-like receptor 4(Lps) (Tlr4 Mouse)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed Proline 712 to Histidine. Native signal p…

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pRevTRE MKL1 S449A/T450A/S454A

Plasmid

#19847

Depositor

Ron Prywes

Publication

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
Strain HK022 JQ929581

Bacterial strain

#38204

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Bacterial resistance

Kanamycin

Species

Synthetic

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-KHK

Plasmid

#23586

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KHK (KHK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTripZ EV

Plasmid

#209071

Purpose

DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control

Depositor

Matthew Oser

Publication

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Insert

stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pJT302 Ec03 Lenti pEF-attB LSR GFP

Plasmid

#184945

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Publication

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-WAVE1/SCAR [K91/36R]

Plasmid

#128629

Purpose

Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neurexin-1-Beta [N170A/1.1R]

Plasmid

#114530

Purpose

Mammalian Expression Plasmid of anti-Neurexin-1-Beta (Human). Derived from hybridoma N170A/1.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neurexin-1-Beta (Homo sapiens) recombinant mouse monoclonal antibody (NRXN1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.1b K+ channel [N16B/8R]

Plasmid

#114511

Purpose

Mammalian Expression Plasmid of anti-Kv3.1b K+ channel (Rat). Derived from hybridoma N16B/8.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.1b K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Publication

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSA3

Plasmid

#52263

Purpose

Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEF myc Gal CDK9 (P#1475)

Plasmid

#14631

Depositor

Matija Peterlin

Publication

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cdk9 (CDK9 Human)

Tags

Gal and myc

Expression

Mammalian

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFosill-1

Plasmid

#89695

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Publication

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET B-DKAR

Plasmid

#14881

Depositor

Alexandra Newton

Publication

Kunkel et al J Biol Chem. 2007 Mar 2. 282(9):6733-42.

Insert

D kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pRN3P_Kdm5b_MUT

Plasmid

#119767

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

Kdm5b MUT

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-LRP4 (cytoplasmic) [N164/6.1R]

Plasmid

#114542

Purpose

Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KB2

Plasmid

#23533

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KB2 (RPS6KB2 Human)

Use

Gateway Cloning

Mutation

R482Stop

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only