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Showing: 7001 - 7020 of 32649 results

  1. DPAP1-COMP-blac-flag-his

    Plasmid
    #111019
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    cathepsin C, homolog,dipeptidyl aminopeptidase 1 (DPAP1) (PF11_0174 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111019-plasmid-map-sequence-id-224593

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  2. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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  3. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

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  13. RAB25

    Plasmid
    #26163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB25:HPC038-B06:C25773 (RAB25 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. 4IJD

    Plasmid
    #51328
    Depositor
    Cheryl Arrowsmith
    Insert
    4IJD (PRDM9 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pIC79

    Plasmid
    #63063
    Purpose
    Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hNSL1, IMAGE clone 3997280 (NSL1 Human)
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63063-plasmid-map-sequence-id-104378

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  18. pLenti CMV GSE T329A Hygro (w189-4)

    Plasmid
    #22255
    Depositor
    Eric Campeau
    Insert
    Genetic suppressor element #22, T329A
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Threonine 329 is mutated to Alanine (p53 nomencat…
    Available Since
    Dec. 15, 2009
    Availability
    Academic Institutions and Nonprofits only
    22255-plasmid-map-sequence-id-10195

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  19. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid
    #58799
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag and Ha
    Promoter
    T7
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58799-plasmid-map-sequence-id-91094

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  20. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

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Showing: 7001 - 7020 of 32649 results