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pcDNA3.1-BCR-ABL-p210-ΔIDR3-FLAG

Plasmid

#205624

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted IDR3 domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTdelta5cys

Plasmid

#207465

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTdelta5cys (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔFA-FLAG

Plasmid

#205625

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted FA domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔDH-FLAG

Plasmid

#205629

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted DH domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-KD-FLAG

Plasmid

#205626

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

kinase-dead (KD; K271H)

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-Y177F-FLAG

Plasmid

#205627

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

Y177F

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔAB-FLAG

Plasmid

#205628

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted AB domain

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔTK-FLAG

Plasmid

#205630

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted tyrosine kinase (TK) domain

Promoter

CMV

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H262A

Plasmid

#202782

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H262A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-T244A

Plasmid

#202781

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

T244A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-Y209A

Plasmid

#202779

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y209A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-Y210A

Plasmid

#202780

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y210A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K208D

Plasmid

#202785

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K208D

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-C264A

Plasmid

#202778

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

C264A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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