-
Plasmid#53340PurposeExpresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertEGF (EGF Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 KP.2
Plasmid#223273PurposeEncodes SARS-CoV-2 spike KP.2 for pseudovirus productionDepositorInsertSARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneUseTags6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
PEW-pEF1A-inactEGFP
Plasmid#212009PurposeCleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transductionDepositorInsertInactivated eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertring finger protein 31 (RNF31 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cre
Plasmid#34564PurposeRetroviral expression of CreDepositorInsertCre
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK391 (mAID-BSD)
Plasmid#121192PurposeC-terminal taggingDepositorInsertmAID-BSD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)
Viral Prep#105545-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHITagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0
Plasmid#208726PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMIA10.7
Plasmid#214451PurposeBxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.DepositorInsertsTet-On 3G
PuroR
TRE3G CopGFP
UseBxbi donorTagsPuroR, T2A, and Tet-On 3GExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV9)
Viral Prep#83896-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2olig
Plasmid#101222PurposeCry2olig(E490G)DepositorInsertCry2olig
UseLentiviralTagsExpressionMammalianMutationE490GPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only