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pAK22

Plasmid

#101741

Purpose

encoding two copies of T. thermophilus SecG for expression of SecYEG complex

Depositor

Tomoya Tsukazaki

Article

Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do

Insert

Thermus thermophilus HB8 SecG x 2

Tags

NONE

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1 GW

Plasmid

#79750

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nat Genet. 2006 Jan;38(1):63-7. Epub 200

Type

Empty backbone

Tags

none

Expression

Plant

Promoter

ProCDKA;1

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Ppms028(JBEI-11601)

Plasmid

#87958

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE144

Plasmid

#79448

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE148

Plasmid

#79450

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE151

Plasmid

#79451

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE160

Plasmid

#79452

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE164

Plasmid

#79454

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE165

Plasmid

#79455

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE166

Plasmid

#79456

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE169

Plasmid

#79459

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE2

Plasmid

#79441

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL6

Plasmid

#72990

Purpose

RBS_M3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL7

Plasmid

#72991

Purpose

RBS_M5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL9

Plasmid

#72993

Purpose

RBS_L2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L2 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL10

Plasmid

#72994

Purpose

RBS_L3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL12

Plasmid

#72996

Purpose

RBS_L5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-BBa_B0012

Plasmid

#72997

Purpose

BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0012 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S2P21

Plasmid

#72999

Purpose

L3S2P21 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S2P21 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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