We narrowed to 20,426 results for: REN

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A

Plasmid

#172206

Purpose

Expresses KIF1A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

Plasmid

#189752

Purpose

Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCXN2-BCL6 (CXN-BCL6)

Plasmid

#40346

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

BCL6 (BCL6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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scOrange chromoprotein

Plasmid

#117840

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, scOrange

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNPK-NLS-Vika

Plasmid

#79969

Purpose

expresses human codon-optimized Vika-recombinase in mammalian cells

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

human codon-optimized Vika sequence, NLS tagged

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pR26

Plasmid

#127372

Purpose

Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Promoter

tight TRE promoter

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Tags

T2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVTNT PINK1 C-myc

Plasmid

#13314

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pR26-CMVconst

Plasmid

#127373

Purpose

Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Promoter

CMV enhancer/promoter

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α wt-pBabe-Puro

Plasmid

#26055

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein

Plasmid

#117842

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LAMP1

Plasmid

#110948

Purpose

Expresses LAMP1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R249S

Plasmid

#22935

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R249S Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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