We narrowed to 31,789 results for: puromycin

1. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-UCHL1

   Plasmid

   #22563

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   UCHL1 (UCHL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP Turquoise2-Syntaxin17

   Plasmid

   #89938

   Purpose

   Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

   Insert

   human Syntaxin 17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM705 Gsi (Ser)

   Plasmid

   #109352

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cys at position 391 to Serine (Pertussis …

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM705 Gst (Ser)

   Plasmid

   #109354

   Purpose

   GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gst chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM705 Gso (Ser)

   Plasmid

   #109353

   Purpose

   GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gso chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-puro largeTcDNA

   Plasmid

   #14088

   Depositor

   William Hahn

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   Large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-miniTurbo-V5-CDK7_IDG-K

   Plasmid

   #158194

   Purpose

   Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK7 (CDK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF05J

   Plasmid

   #42941

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Negative control Functionally Dead Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   ORF1 R261A, R262A

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pXPR_050

    Plasmid

    #96925

    Purpose

    lentiviral expression of gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLKO.1-UPF1-3'UTR

    Plasmid

    #136036

    Purpose

    UPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK243 (Tet-OsTIR1-PURO)

    Plasmid

    #72835

    Purpose

    Expresses OsTIR1 under the control of the Tet promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-Tet-OsTIR1-PURO-AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-FAST-p10

    Plasmid

    #89152

    Purpose

    Express p10-FAST in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    NBV p10-FAST
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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